Titan gel lipoprotein electrophoresis system

agarose gel electrophoresis system / bench-top SPIFE Touch™ quality control reagents / for electrophoresis / serum / lipoprotein TITAN III LipoTrol.
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Of these systems, only RIA used an apo a detection antibody not directed against the K4—2 epitope on apo a ; except for a: B assays, all other tested systems used polyclonal or monoclonal antibodies, which are considered apo a size-sensitive. However, the parallelism results for these systems indicate that there was negligible isoform-related concentration bias for samples containing isoforms from 20 to 24 apo a K4 repeats in size.

Therefore, although some commercial Lp a calibrators appear not to be commutable between different assays, others are compatible within the majority of systems and show similar reaction characteristics to serum in addition to specified precision and linearity requirements.

In addition, assay reagent formulations should be optimized to enable better linearity of both standard and serum samples and at the same time produce parallelism between these materials. Of the Lp a assays tested, it appears that there is no one particular method type that is better or worse than another; acceptable analytical precision, linearity, and parallelism were shown by individual test systems from all method groups.

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The decision as to which of the current assays are most accurate and least biased towards Lp a isoform size will be determined in Phase 3 of the standardization project, when fresh-frozen serum samples with Lp a values assigned against the selected method are analyzed. From the results of Phase 1, it seems highly likely that the suboptimal analytical performance of a large number of existing Lp a assay systems has contributed to the poor comparability of values between methods to a greater extent than recognized in previous Lp a surveys.

Consequently, it is not surprising that, even after correction of Lp a values for calibrator differences between systems, there was not a closer comparison of Lp a values for the 40 test systems. In some assay systems, unexpectedly low values for the frozen serum control FSC B, against which all values were referenced, have partly contributed to poorer harmonization results. This closer comparability of values further reinforces the requirement for Lp a assays to meet specified precision and linearity performance standards.

The final, unequivocal confirmation of successful Lp a harmonization will take place in Phase 3 of the standardization project, when a series of samples of varying Lp a concentration and isoform composition are analyzed in assay systems that have been optimized for analytical performance and uniformly calibrated against the selected secondary reference material for Lp a. The evaluation of manufactured calibrator materials in existing Lp a test systems has shown some calibrators to be comparable with serum samples in terms of precision, linearity, and parallelism assay characteristics.

Results from optimized assays indicated that Lp a values agreed closely between methods for some calibrators but deviated widely for others, indicating their lack of commutability across all Lp a assay systems. Therefore, any future selection of a manufactured material as a reference material for Lp a must consider its analytical performance, harmonization effect, commutability, and stability properties.

Most importantly, monitoring of the long-term stability of such a material is essential because sample degradation leading to changes in recognition of the apo a epitope by the antibody may affect Lp a measurement in some assay systems. In combination with these requirements is the prerequisite that proposed reference materials be quantitatively tested in optimized assay systems.

The development of a common calibrator and choice of a selected Lp a method for value assignment are currently in progress in Phase 2 of the standardization project. In summary, an evaluation of 40 Lp a test systems was undertaken to determine the analytical performance of existing Lp a assays in use worldwide. From the Phase 1 results, it is apparent that a significant number of the assays were not optimized i. More work on the part of the manufacturers is required to address these concerns.

The IFCC WG Lp a recommends that a similar evaluation consisting of method assessment and comparison of samples be done when selecting an Lp a method to be used in large clinical trials or epidemiological studies. Finally, the desired harmonization of Lp a assays will require the use of a universal standard, and results presented here indicate the possibility of commercial Lp a materials being a satisfactory source of secondary reference material for Lp a.

We gratefully acknowledge the following diagnostic companies, which provided the proposed Lp a reference materials for this study: WG Lp a: Diagnostic Companies: Beckman Instruments Inc. B assays. Missing data: Commercial sources of detection antibodies addresses listed in Acknowledgments included Beckman Instruments, Inc. In addition, Daiichi Pure Chemicals Co. Skip to main content.

Other Lipids and Lipoproteins. Jillian R. Kostner , Ikunosuke Sakurabayashi , Armin Steinmetz. Published August Abstract A secondary reference material for lipoprotein a is required to standardize the measurement of lipoprotein a in clinical laboratories worldwide.

QuickGel Lipoprotein

Materials and Methods participating laboratories Thirty-three laboratories from 12 countries, including the laboratories of 6 working group members and 26 diagnostic companies, participated in Phase 1 of a study designed by the IFCC WG Lp a to standardize assays for the measurement of Lp a. Lp a assay systems Among the 40 test systems evaluated in Phase 1, 7 different Lp a methods were used. View this table: View inline View popup. Table 1.

Lp a methods used in study. Table 2. Table 3. Figure 1. Figure 2. Figure 3. Table 4.

Table 5. Discussion The inability to compare absolute Lp a concentrations between different clinical studies and the large intermethod variation in values, documented by several surveys, are mainly attributable to the lack of a universal Lp a standard and calibrators traceable to this material. Acknowledgments We gratefully acknowledge the following diagnostic companies, which provided the proposed Lp a reference materials for this study: Participating laboratories: A new serum type system in man—the Lp system.

Acta Pathol Microbiol Scand ; Clin Genet ; 6: Lipoprotein Lp a and the risk for myocardial infarction. Atherosclerosis ; The association between serum Lp a concentrations and angiographically assessed coronary atherosclerosis. Dependence on serum LDL levels.

Lp a lipoprotein as a risk factor for coronary heart disease and cerebral infarction. Nature ; International Lp a standardization. Sakurabayashi I. Results of the survey for the correction of inter-laboratory variations by a unified reference material.

Electrophoresis reagents / hemoglobin - Titan III-H - Helena Laboratories

Jpn J Clin Pathol ; Lipoprotein a: Clin Biochem Rev ; A comparative study of six commercial lipoprotein a assays in seventeen laboratories within the British Isles. Ann Clin Biochem ; Berg K. Confounding results of Lp a lipoprotein measurements with some test kits. Clin Genet ; Determination of lipoprotein a: Standardization of Lp a measurements. Couderc R. Immunodosage de la Lp a. Etude de la conservation et essai de standardisation. Paris, France: Tate J. Lipoprotein a standardisation within Australia and New Zealand. Kostner GM. Standardization of Lp a assays [Letter].

Clin Chim Acta ; Effect of the number of apolipoprotein a kringle 4 domains on immunochemical measurements of lipoprotein a. Clin Chem ; Lipoprotein a levels in patients receiving renal replacement therapy: Am J Kidney Dis ; Kostner GM , Grillhofer H. The interaction of Lp a with normal and LDL-receptor-deficient human skin fibroblasts.


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Lp a glycoprotein phenotypes. Inheritance and relation to Lp a -lipoprotein concentrations in plasma. J Clin Investig ; Molecular definition of the extreme size polymorphism in apolipoprotein a. Hum Mol Genet ; 2: Immunoturbidimetric determination of lipoprotein a: Polymorphonuclear cells isolated from human peripheral blood cleave lipoprotein a and apolipoprotein a at multiple interkringle sites via the enzyme elastase. J Biol Chem ; Apolipoprotein a kringle IV repeat number predicts risk for coronary heart disease. Arterioscler Thromb Vasc Biol ; Previous Next.

Back to top. In this issue Vol. Table of Contents Index by author. International Federation of Clinical Chemistry standardization project for the measurement of lipoprotein a. Phase I. Evaluation of the analytical performance of lipoprotein a assay systems and commercial calibrators. Clinical Chemistry Aug , 44 8 ;.


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    7. Share This Article: Article Alerts. It has been widely used in identification of monoclonal gammopathies. Immunoelectrophoresis IEP combines two techniques, electrophoresis and immunodiffusion. In this two-part procedure, proteins are first separated by electrophoresis. Then antisera, complementary to the proteins under study, are applied to the gel and allowed to diffuse.

    When a favorable antigen to antibody ratio exists, a precipitin arc forms on the gel. Both thick and thin gels are available for performing immunoelectrophoresis. The thick agarose, on a rigid plastic backing for ease of handling, requires a larger volume of antisera and patient samples, and forms a dense precipitin arc easily interpreted and photographed without staining the gel.

    The thin agarose gel, on a flexible plastic backing, provides economical use of antisera and lends itself to staining and drying of the gel for a permanent record. Each gel has a plastic lid and a reusable plastic pouch. Results may be interpreted without staining. Gels require 4 mL of sample and 75 mL of antisera. The well position is 17 mm from the cathode end of the trough. The recommended migration distance is 40 mm. Gels require 4 mL of sample and mL of antisera. The well position is 23 mm from the cathode end of the trough.